ISO 11267:2014 pdf download

ISO 11267:2014 pdf download.Soil quality — Inhibition of reproduction of Collembola (Folsomia candida) by soil contaminants
1 Scope
This International Standard specifies one of the methods for evaluating the habitat function of soils and determining effects of soil contaminants and substances on the reproduction of Folsomia candida Willem by dermal and alimentary uptake. This chronic test is applicable to soils and soil materials of unknown quality, e.g. from contaminated sites, amended soils, soils after remediation, industrial, agricultural or other sites of concern and waste materials. Effects of substances are assessed using a standard soil, preferably a defined artificial soil substrate. For contaminated soils, the effects are determined in the soil to be tested and in a control soil. According to the objective of the study, the control and dilution substrate (dilution series of contaminated soil) are either an uncontaminated soil comparable to the soil to be tested (reference soil) or a standard soil (e.g. artificial soil). This International Standard provides information on how to use this method for testing substances under temperate conditions. The method is not applicable to volatile substances, i.e. substances for which H (Henry’s constant) or the air/water partition coefficient is greater than 1, or for which the vapour pressure exceeds 0,013 3 Pa at 25 °C. NOTE The stability of the test substance cannot be ensured over the test period. No provision is made in the test method for monitoring the persistence of the substance under test.
4 Principle
The effects on reproduction of 10 d to 12 d old Collembola (Folsomia candida) exposed to the soil to be tested are compared to those observed in a control soil. If appropriate, effects based on exposure to a test mixture of contaminated soil and control soil or a range of concentrations of a test substance mixed into control soil are determined. Test mixtures are prepared at the start of the test and are not renewed within the test period. The Collembola are incubated until offspring (F 1 ) emerge from eggs laid by mature adults, and the number of offspring is determined. Usually offspring emerge within 28 d in control experiments. The results obtained from the tests are compared with a control or, if appropriate, are used to determine the concentrations which cause no effects on mortality and reproduction (NOER/NOEC) and the concentration resulting in x% reduction of juveniles hatched from eggs compared to the control (ERx/ECx, 28 d) respectively. If testing a concentration series, all test dilutions/concentrations above the LOER/LOEC have a harmful effect equal to or greater than that observed at the LOER/LOEC.