ISO 23919:2020 pdf download

ISO 23919:2020 pdf download.Cigarettes — Determination of ammonia in cigarette mainstream smoke using ion chromatography
1 Scope
This document specifies a method for the quantification of ammonia by ion chromatography in mainstream smoke using ISO 3308 smoking parameters. This method is applicable to cigarettes with ammonia yields between 1 µg/cigarette and 30 µg/ cigarette. It is not applicable for the determination of ammonia in dark-air cured cigarettes.
2 Normative references
The following documents are referred to in the text in such a way that some or all of their content constitutes requirements of this document. For dated references, only the edition cited applies. For undated references, the latest edition of the referenced document (including any amendments) applies.
ISO 3308, Routine analytical cigarette-smoking machine — Definitions and standard conditions
ISO 3402, Tobacco and tobacco products — Atmosphere for conditioning and testing
ISO 4387, Cigarettes — Determination of total and nicotine-free dry particulate matter using a routine
analytical smoking machine
ISO 8243, Cigarettes — Sampling
3 Terms and definitions
No terms and definitions are listed in this document.
ISO and IEC maintain terminological databases for use in standardization at the following addresses:
— ISO Online browsing platform: available at https:// www .iso .org/ obp
— IEC Electropedia: available at http:// www .electropedia .org/
4 Principle
Ammonia is collected by passing the mainstream smoke of cigarettes through either
a) a glass fibre filter pad as specified in ISO 3308 followed by impinger traps containing dilute sulphuric acid (trapping system 1 in the document), or
b) an impregnated glass fibre filter pad followed by an untreated glass fibre filter pad (trapping system 2 in the document).
The glass fibre filter pad is extracted either with the impinger solutions (trapping system 1) or with dilute hydrochloric acid (trapping system 2). The obtained solutions are analysed by ion chromatography using an external standard calibration.
5 Apparatus
The usual laboratory apparatus for use in preparation of samples, solutions and standards and, in particular, the following.
5.1 Routine analytical cigarette-smoking machine, complying with the requirements of ISO 3308.
5.2 Impinger trapping system, capable of being connected in series.
5.3 High performance liquid chromatography system, consisting of a conductivity detector and conductivity suppressor, an eluent degassing unit, a gradient pump, an autosampler with sampling loop and cooling unit, a data collection system.
5.4 Cation exchange analytical column, e.g. Dionex TM IonPac 2) CS16 IC or equivalent.
5.5 Disposable guard column, e.g. Dionex TM IonPac CG16 2) or equivalent.
5.6 Analytical balance, suitable for measuring to the nearest 0,1 mg.
5.7 Glassware, Erlenmeyer flasks of appropriate volumes with ground glass stoppers.
5.8 Polypropylene, tubing, volumetric flasks (25 ml, 50 ml, 100 ml and 1 l).
5.9 Mechanical pipettes with disposable plastic tips.
6 Reagents
All reagents shall be at least of analytical reagent grade.
6.1 Ammonium sulfate [(NH 4 ) 2 SO 4] > 99 % purity.
6.2 Sulphuric acid (H 2 SO 4 ) > 96 % purity (for trapping system 1).
6.3 Hydrochloric acid (HCl) > 36,5 % to 38 % purity (for trapping system 2).
6.4 Methanesulphonic acid (MSA) > 99 % purity.
6.5 Ethanol > 99 % purity.
6.6 Deionised water, with a resistivity > 18 MΩ·cm at 25 °C.