BS ISO 17244:2015 pdf download.Water quality — Determination of the toxicity of water samples on the embryo-larval development of Japanese oyster (Crassostrea gigas) and mussel (Mytilus edulis or Mytilus galloprovincialis)
1 Scope
This International Standard specifies a method for determining the effects of chemical and aqueous samples on the embryo-larval development of marine bivalves. It allows the determination of the concentration levels that result in an abnormality in embryo-larval development. This test is suitable for salinity ranges between 20 and 40 for mussels and between 25 and 35 for oysters. This method applies to
— chemical substances and preparations,
— marine and brackish waters,
— streams and aqueous effluents (urban, agricultural, industrial effluents, etc.) as long as the salinity is adjusted and/or dilution is limited so that the aforementioned salinity ranges are respected, and
— aqueous extracts (pore water, elutriates, eluates, and leachates) from sediments and petroleum products.
2 Normative references
The following documents, in whole or in part, are normatively referenced in this document and are indispensable for its application. For dated references, only the edition cited applies. For undated references, the latest edition of the referenced document (including any amendments) applies.
ISO 5667-16, Water quality — Sampling — Part 16: Guidance on biotesting of samples
ISO 14442, Water quality — Guidelines for algal growth inhibition tests with poorly soluble materials,volatile compounds, metals and waste water
4 Principle
This biotest assesses the effects of chemicals and aqueous environmental samples on the embryo-larval development of marine bivalves under static conditions. The exposure is performed from fertilized eggs to D larvae. This static test aims to determine the concentration level (EC x ) which results in abnormalities for x % of exposed larvae in 24 h for the Japanese oyster, also named Pacific oyster (Crassostrea gigas), and in 48 h for the mussel (Mytilus edulis or Mytilus galloprovincialis). Several parameters can be assessed on the abnormal larvae: alteration of the shell (hinge is not straight, unequal, or incomplete valves), hypertrophy of the mantle, delayed or stopped embryonic development, and finally, death. The results are expressed as EC x (EC 20 or EC 50 ). The lowest observed effect concentration (LOEC) and no observed effect concentration (NOEC) can also be determined. NOTE This method can be applied to other species of bivalves (e.g. C. virginica). Nevertheless, the test conditions have to be defined to reach the validity criteria of the standard.
BS ISO 17244:2015 pdf download
