BS EN 1276:2019 pdf download.Chemical disinfectants and antiseptics一 Quantitative suspension test for the evaluation of bactericidal activity of chemical disinfectants and antiseptics used in food, industrial, Chemical disinfectants and antiseptics
2 Normative references
The following documents are referred to in the text in such a way that some or all of their content constitutes requirements of this document. For dated references, only the edition cited applies. For undated references, the latest edition of the referenced document (including any amendments) applies.
EN 12353，Chemical disinfectants and antiseptics – Preservation of test organisms used for the determination of bactericidal (including Legionella), mycobactericidal, sporicidal, fungicidal and virucidal (including bacteriophages) activity
EN 14885:2018, Chemical disinfectants and antiseptics – Application of European Standards for chemical disinfectants and antiseptics
3 Terms and definitions
For the purposes of this document, the terms and definitions given in EN 14885:2018 apply.
ISO and IEC maintain terminological databases for use in standardization at the following addresses:
● IEC Electropedia: available at http://www.electropedia.org/
● ISO Online browsing platform: available at https:/ /www.iso.org/obp
4 Requirements
The product shall demonstrate at least a 5 decimal logarithm (g) reduction (3 lg for handwashes) when diluted with hard water (5.2.2.7) or- in the case of ready-to-use products – with water (5.2.2.2) and tested in accordance with Clause 5 under simulated clean conditions (0,3 g/l bovine albumin solution- 5.2.2.8.2) or simulated dirty conditions (3 g/l bovine albumin solution – 5.2.2.8.3) according to its practical applications and under the suitable test conditions as described in 5.5.1.1, Tables 1 and 2 here below.
5 Test method
5.1 Principle
5.1.1 A sample of the product as delivered and/or diluted with hard water [or water for ready-to-use products with the exception of handwash products whose first dilution is done in hard water (5.4.2]) is added to a test suspension of bacteria in a solution of an interfering substance. The mixture is maintained at the chosen test temperature for the adopted contact time. At the end of this contact time, an aliquot is taken, and the bactericidal and/or the bacteriostatic activity in this portion is immediately neutralized or suppressed by a validated method. The method of choice is dilution-neutralization. If a suitable neutralizer cannot be found, membrane filtration is used. The numbers of surviving bacteria in each sample are determined and the reduction is calculated.
5.1.2 The test is performed using Pseudomonas aeruginosa, Escherichia coli, Staphylococcus aureus and Enterococcus hirae as test organisms. For temperatures≥40 °C only Enterococcus faecium shall be used.
BS EN 1276:2019 pdf download
